we have modified the traditional method of molecular cloning. With this modified method, the cloning efficiency can be obviously improved. Here is the paper about this method.
http://www.ncbi.nlm.nih.gov/pubmed/24613256
It may be especially valuable for some challenging cloning, such as blunt-end cloning, single-enzyme-digested sticky-end cloning, and some double digested DNA cloning.
If you have trouble during molecular cloning, maybe you can have a try. If you have a publication, please do not forget to cite our paper. Thank you before hands.