virion

This probably isn't the best forum for technical questions, but oh well.
 
Most of the cDNAs will not be full length. If you've just got a kit with random hexamer and oligodT primers, you'll have a mix of "all" cDNAs. But you could amplify selectively too if sequencing a single gene is your goal.
 
If you need to amplify an entire gene, don't just use some random primers generated in the middle of the reading frame (was the web app doing it for qPCR?). As you seem to suggest, you'll only get the piece in the middle. 5000 kb is an extremely long PCR. There's no reason you can't just do it in pieces and sequence those. Unless you're using a polymerase like pfu or phusion, you're going to introduce mutations when amplifying, which would be counterproductive for your purposes.

Yikes. This is the last thing that I would do if I were in his situation. This would ensure that the gossip would spread.

Because he really wants to be further associated with the topic of rape...

and in regards to "feminists," I'm talking about women who will do and say anything to justify their position of victimization in a -- so they want to emphasize -- "patriarchal society". so they jump on the bandwagon when cases like this leak, as they get to suspect a man for being oh-so-evil! they tend to disregard the fact that men 1) have their unique feelings and insecurities and 2) women can make up stories precisely to take advantage of a "victim" position. these "feminists" make me ashamed to be one myself.

What is this supposed to mean?

You don't even know the full story yet you're willing to blame this on "feminists"? Disgusting. You're what's wrong with rape culture.

I'm so sorry to hear about this! I think this story shows how our society (especially self-righteous "feminists") is so willing to villainize men to the point that men are dehumanized. i really think men have feelings equally as women do.

i don't know what to tell you except to keep insisting on your innocence and tell them that you'd be in jail, instead of in academia, had you really been guilty?

Hello,

I am not sure if this is the right place to post this, but I have a potentially silly question regarding the table lamps in the Loker Reading room of Widener Library here at Harvard.
How do I turn those lamps on?!

I think you're right that few people will be familiar with the program specifically (I'm not familiar with the field and their typical admissions process).

I would speculate that this kind of bulk screening would not be necessary for a program receiving fewer applications. I doubt the big programs do it because they're 'super elite'. It's more likely that they simply can't look at every application in detail.

I dunno - I'd be super nervous too at that age attending an overseas program.

I'm quite used to the condescending tone of your post. I find it quite funny that the only ones who ever do try to minimize the things I have accomplished are other minorities. "Oh, you make it sounds easy" (it wasn't). "Not everyone is like you" (aren't I a special snowflake?). Please. People on here have accomplished something that most people will never be able to accomplish (i.e. graduate from college) and are seeking to go further... no one who is on their way to a getting a graduate degree should think of themselves as "oppressed."

I was raised in a country where tens of thousands of people, many of whom were in college, were kidnapped and killed by the government. A generation of intellectuals, gone. THAT is oppression.

Perhaps a little world perspective would show just how NOT oppressed Americans really are.

What I said is that people stress themselves out NEEDLESSLY over how things "are going to be," and how to deal with "being a minority." When you encounter real discrimination, deal with it then. But trying to "deal with it" before it even happens is a pointless exercise. It's like freaking out about whether the new group of people you're about to meet is going to like you, and trying to think of ways of dealing if they don't. It seems to me a much better use of my time would be to address any instances when they occur... not try to plan for them assuming they will happen.

My first brush with discrimination happened not long after I arrived at the US at age 16... despite having good grades from K-10, my high school counselor discouraged me from applying to four-year universities. She said my education in Latin America had not been a good as if I had lived in the US... for instance, she dismissed my A's in Physics saying that the Physics I had studied was not the same as what was taught here. Then she suggested a vocational school so I could "help my family." Maybe cosmetology?

I ignored her, applied to several highly-ranked universities, and got into every single one. And that's when I realized that I was going to ignore counselors and forge my own path. I also told myself I would NEVER let anyone else define me... she defined me as a minority, and thus someone inferior. Never again.

Although I can easily be l labeled as "Latina," I don't identify myself as such. I am very proud of my heritage and I'm very much in touch with the culture I grew up in, but I'm an American. I don't see myself as a member of a minority, and I don't feel I have to prove myself BECAUSE I'm a "Latina." I already work hard because I set very ambitious goals for myself... and it's not worth it to stress myself out over whether others will have some preconceived notion based on how they wish to define my identity.

I think sometimes people who are members of minorities pay way too much attention to others' perceptions of one's minority status (whether it be race, gender, sexual orientation, nationality, religion, etc.) and set themselves up for additional stress... your "status" becomes yet another hurdle to jump through, and in my experience as a student and as an educator, it often is a hurdle of our own creation.

Just go in there and do the absolute best you can do. Don't ever let them see you as a "black female" and treat you as such... Let them see you as the brilliant student and scholar you are, and demand the respect you have earned by being so darned awesome.

That's always the refrain isn't it: can't make your point be accepted as fact, just label the person you disagree with a troll and feign ignorance on how the conversation took a nasty tone. Well done. Round and round she goes.

I'd say "Physician, heal thyself. . ." but that's just too obvious I think -

If you say so. This thread has already derailed enough, no way am I taking this flame bait; least of all your ludicrous chicken sandwich ipso facto argument. . .



Thank goodness there are people who can actually do math in this world who dismiss such outlandish comments without actually doing some research first (population the competition is drawing from, historical proportion of men to women in the field of interest - yanno, the pesky stuff).

If it turns out that this is a significant difference, then yes you can dance a jig at the gender bias (something tells me you haven't done that).



Two people find that comment disgusting (and not simply churlish or immature): you (gee I wonder why) and the tightwad it was directed to. Good game.



Sorry, I was merely addressing personal snark with personal snark. Feel free to continue thread combing for posts to Rep-Bomb. It's a hoot.

And your disgusting comment helps make sure it stays not non-existent.



Surely you know that it’s acceptable webiquette to address the content of a post, not its writer. Surely, though, if you choose to violate that convention, you’d at least learn what it is that a linguistics PhD does so as not to embarrass yourself with your ignorance.

I will spell it out: The chick fil a supporters are bigots. Lots of smart people are gay and don't want to live where their neighbours are actively trying to take away their rights. Those people will leave and move to tolerant places. And there are other, non-gay smart people who won't want to live surrounded by bigots either, so they'll stay away too.

And a competition that produces 19 awards to men and none to women is plainly sexist (see "disparate impact"). Even the minister in charge admitted things had gone wrong somewhere.

I can think of a couple things; perhaps you've been in school too long. You can't see the forest for the smooth milky thighs. . .

Pretty non-existent is not non-existent. Surely, to a linguistics PhD, I didn't really need to lay that out.

I'm sorry but that is a load of bullsh*t. Not your demographics - one need only stumble into a computer lab random times during the day to confirm this - but the incredibly faulty connection that "Hey it's full of all dudes. . .definitely has the undercurrent of sexism in it!" - relative or otherwise. This is still academia; unless the OP is going to school in Iran or something gender/racial biases are pretty non-existent.



Jesus. And you're in Social Psychology too. God help us -

As for "insisting" on the department - I was simply curious if the OP would bite. They didn't. Coupled with the previous info, I'm simply leaning more towards "less insidious than a normal person would think - " in terms of the tenor of the conversation the OP heard.

Of the fellow interviewees I met during my round of interviews this year, many who weren't current undergrads were working industry jobs (quite a few RA's in academia as well). I myself worked as a research assistant at a university lab which publishes, etc, but does not have a standard structure (no graduate students and basically never post docs). So I received a few questions from interviewers trying to make sure I was familiar with standard academic lab environment, had indirect exposure to 'grad school life', and so on. As an undergrad, I worked in such labs. This always seemed to put the interviewer at ease.

So, I would say that it partially depends on your previous experience. The schools I interviewed with generally made a big deal about the fact that they are aware that no, most PhD graduates won't get traditional jobs in academia and yes, they're here to prepare us for other post-graduate job we may be seeking. So if the admissions committee reading your application has this "modern" type of attitude, the fact that you know you want to go into industry and have relevant experience to that may work for you. It's also possible that an old-timer (or whoever) that perceives a stigma associated with that type of work will read your application. But generally I think they'll like to see someone who knows what they want.

This doesn't address the issue of networking you mentioned. If you would be working with people in the program you eventually want to join, it's an opportunity that would be difficult to pass up.

Hopefully you're offered both jobs But if, for instance, you only get the biotech job there is absolutely no shame in that and you'll thank yourself in grad school for the extra money you've been able to set aside.

I usually notice a change in email signature over time.

Dr. Franklin Lastname
Franklin Lastname
Franklin
Frank

You don't like your main advisor, think he "bashes" only you, consider that he doesn't care about your project, call him "lazy", feel ignored... yet your main concern is that you quitting is going to "hurt or embarrass him"??

What are you studying? It's important as a PhD student to be able to take some initiative and work independently, and that includes compiling your own bibliography. Your advisor can add to it, but the fact that he didn't hand you a bibliography is not a good reason to write nothing. You say you don't have a bibliography, but what do you have after your first year of PhD? I personally find it strange that even though you have three advisors, and there are several more advanced grad students in your department, you still have no clue how to proceed with writing a draft of the first part of your thesis (the layout of the draft should be your least concern).



I think he's quite demanding of you for someone who doesn't care about your PhD and your project. To avoid alienating the people in your department, and potentially turning some professors against you, my suggestion would to sweep the "poor me" attitude under the rug. Your concerns about your work and your needs from an advisor (not those of your peers), would be best discussed face-to-face, calmly and respectfully, either with your main advisor, with that one advisor that you do like, or with another professor in the department with whom you feel more comfortable discussing your issues; I would avoid whining to the other PhD students if I were the only one having a strained relationship with my advisor.

Out of curiosity, among the two PhD programs that accepted you, is the one you're currently enrolled in the one that best matched your research interests?

Excellent contribution. Guessing you're building up your post count to drop a bunch of spam for the url listed as your website.

If any one got an acceptance from University of Southern California neuroscience program kindly let me know.Pretty please

sOMEBODY NEEDS TO ANSWER

These are all top-tier schools and you will be able to find any academic job coming out of any of these schools. The only obvious difference is location, which might matter if you're looking for an industry job (eg. a boss at a company in LA may prefer to higher CalTech grads...). But here are the general differences in location, as I understand them:

CalTech - LA - California has a great track record for making millionaires and dreams come true. It's an opportunistic place, and Pasadena, CA is no different. It is one of the richest towns per square foot. How does a tiny town like that host the Rose Parade every year? $$$. But even though you'll probably find students with the best work-life balance here, CalTech is extremely competitive.

UChicago - Chicago - You'd think a school in the midwest would be very friendly, but UChicago is in a league of its own. Often labeled as an "honorary Ivy," UChicago is the most desired school in the mid-west. For that reason, it's very competitive and you won't see much collaboration. But Chicago is one of the most vibrant cities in the world.

Yale - The northeast region of the U.S. has the highest density of intellectuals. For that reason, expect the most competitive environment and be prepared to fend for yourself. Collaboration is virtually inexistant. Many Ivy Leaguers describe an extremely unwelcoming, isolating, and socially exclusive community that is most common at Harvard and Yale.